Other
Part:BBa_K1903018:Design
Designed by: Rafael Montenegro Marín Group: iGEM16_TEC-Costa_Rica (2016-10-17)
Golden Gate Adapter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 107
Illegal BsaI.rc site found at 1
Design Notes
This part is designed to be a golden gate adapter. We added a random sequence into the BsaI cut sites to make a sequence easy to detect after a PCR reaction and leading the enzyme digestion. For further design a good option is to change the random sequence with a RFP o GFP as a device, using J23101 for example, to have a selection in the transformation.
Source
The restriction site sequence was obtained from NEB enzyme finder.