Other

Part:BBa_K1903018:Design

Designed by: Rafael Montenegro Marín   Group: iGEM16_TEC-Costa_Rica   (2016-10-17)


Golden Gate Adapter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 107
    Illegal BsaI.rc site found at 1


Design Notes

This part is designed to be a golden gate adapter. We added a random sequence into the BsaI cut sites to make a sequence easy to detect after a PCR reaction and leading the enzyme digestion. For further design a good option is to change the random sequence with a RFP o GFP as a device, using J23101 for example, to have a selection in the transformation.

Source

The restriction site sequence was obtained from NEB enzyme finder.

References